Journal: Stem Cell Research & Therapy
Article Title: The Rotary Cell Culture System increases NTRK3 expression and promotes neuronal differentiation and migratory ability of neural stem cells cultured on collagen sponge
doi: 10.1186/s13287-021-02381-y
Figure Lengend Snippet: The transplantation of NSCs in rats with spinal cord injury. a Spinal cord injuries (with lengths of 3 mm) were inflicted in rats (left). Functional collagen sponge scaffolds with or without cells were precisely placed into the injury site (middle). Samples of the spinal cord tissue following perfused 4% paraformaldehyde fixation (right). b The BBB scores of various groups. Locomotor function recovery in five groups at 1, 2 and 3 weeks after spinal cord injury. c Images of immunofluorescence staining of spinal injury sections at 2 weeks from different groups of SCI rats, stained with antibodies against GFAP (red) and with DAPI (blue). The green signal represents the GFP-labelled transplanted NSCs. Scale bars: 500 m. d Enlarged images of the migrated GFP + cells in the RCCS and RCCS+NT-3 groups. Quantification of the number of migrated cells showing that the NSCs in RCCS+NT-3 group possess the best migratory ability in SCI animals. Scale bars: 250 m. Data represent meanS.D. from three independent experiments. *** p < 0.001
Article Snippet: Cells or frozen sections of Sprague-Dawley (SD) rat spinal cord tissues were fixed with 4% paraformaldehyde and then pretreated with 0.8% Triton X-100 to increase the permeability of the cell membrane.
Techniques: Transplantation Assay, Functional Assay, Immunofluorescence, Staining